In February 1821 Isaiah, together with his brother and Hatzis, the abbot of the Monastery of St. Luke, went to Istanbul, where he met with the Patriarch.
On March 11, he returned and secretly disembarked in Antikyra. He went to the Monastery of St. Luke, where Athanasios Diakos was waiting for him. During the vesper service he and Diakos’ askeri (bunch of men) pledged allegiance to the Revolution flag. He then went to Salona and consulted with Panourgias. Weapons and munitions that arrived from Galaxidi were shared in the houses of the notables.
On March 24, Isaiah blessed the weapons of Panourgias’ men at the Monastery of Prophitis Elias. The notables of Salona Anagnostis Giagtzis, Anagnostis Kehagias and Rigas Kontorigas were also present.
On the 25th of March, Gouras occupied Galaxidi and, on the 28th, Skalchodimos occupied Lidoriki.
On March 26, Isaias arrived again at St. Luke’s monastery, where Diakos was located. A doxology took place, and the next day the armed men left for battle.
On March 27th, the siege of the castle of Salona began, by the rebels of Panourgias, while in the city a “Greek administration” was formed. On the same day, the official beginning of the Struggle was declared by Isaiah of Salona, as he and the other two bishops in the monastery of St. Luke had assembled the rebels that pledged their allegiance to the flag. The Monastery of St. Luke had become the “Hagia Sofia of Roumeli”. The same act took place in the monasteries of St. John the Forerunner and St. Paraskevi, too. He blessed the weapons of those gathered against the Turkish fighters and essentially officially declared the revolution in Boeotia with this symbolic act.
On April 1st, Livadia is occupied.
After the fall of the Castle of Livadia on April 1st and the fall of the Castle of Salona on April 10th (Easter day) that were now at the hands of the Greeks, Isaiah rushed to meet Diakos and Dyovouniotis in Zitouni (Lamia). While fighting in the battle of Alamana, Isaiah, holding the Cross, led the Greek fighters. Though, in the conflict with the troops of Omer Vrioni on the hill of Chalkomata, he was mortally wounded.
The Turks cut off the head of Isaiah’s corpse, and presented it to Athanasios Diakos, together with the heads of Isaiah’s brother and those of other fighters, during his torturous killing by impalement in Lamia. After the death of the Greek General Diakos, the heads, along with the body of the executed man, were thrown into a nearby creek.